PI-based separation of tissue proteins realized with the use of two-dimensional gel electrophoresis or alternatively capillary isoelectric focusing

15.05.2018

Streszczenie

A two-dimensional gel electrophoresis (2D-GE) and capillary isoelectric focusing (cIEF) were performed sub-sequently to analyze protein mixtures from laboratory animal tissues (liver, muscle, and mammary gland). Both tech-niques were compared and evaluated. It was found that cIEF can be a valuable alternative in comparison to 2D-GE. Espe-cially considering the goals associated with the determination of far the separated proteins, cIEF possesses perspective analytical advantages.

Autorzy: Ilona Olędzka, Kamil Włodarski, Piotr Kowalski, J. Grzenkowicz-Wydra, Jolanta Paradziej-Łukowicz, Tomasz Bączek

Źródło: The Open Proteomics Journal 2012; vol. 5, s. 22-28

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