15.05.2018
Seven water-soluble vitamins: thiamine hydrochloride (B1), riboflavin hydrochloride (B2), pyridoxine hydrochloride (B6), calcium pantotenate (B5), ascorbic acid (C ), folic acid (B9), and biotin (H, B7) were separated using the micellar electrokinetic chromatographic (MEKC) method in a single run with the electrolyte solution consisting of 100 mM H3BO3, 5 mM Na2B4O7, and 30 mM sodium dodecylsulphate (SDS). The separation was achieved within 8 min. and the detection was performed at 200 nm. The calibration graphs plotted with six concentrations of each vitamin were linear with the determination coefficient r > 0.999. The method was applied for quantification of vitamins B1, B2, B5, B6, B9, C, and H in novel feed for laboratory animals. The sample preparation involved solid-phase extraction of the vitamins, used prior to the MEKC analysis. The attained precision level was good with the recoveries between 98.4% and 105.6%. The simplicity of the procedure should make it highly useful for feed quality control in husbandry and the animal feed industry.